The isolation and sequence analysis of vasoactive intestinal peptide from a ganglioneuroblastoma.

Abstract

A ganglioneuroblastoma was excised at surgery from a 1-yr-old girl with severe watery diarrhea. The tumor, weighing 1 g, was extracted in trifluoracetic acid and contained 8.3 nmol immunoreactive vasoactive intestinal peptide. The peptide was isolated by affinity chromatography and high pressure liquid chromatography and was found to be identical to porcine vasoactive intestinal peptide by amino acid analysis and microsequence analysis.

• Authors: N W Bunnett, J R Reeve Jr, R Dimaline, J E Shively, D Hawke, J H Walsh
• Journal: The Journal of clinical endocrinology and metabolism (J Clin Endocrinol Metab) Vol. 59 Issue 6 Pg. 1133-7 (Dec 1984) ISSN: 0021-972X [Print] United States
• PMID: 6092409 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Amino Acids
• Vasoactive Intestinal Peptide

Topics

• Amino Acid Sequence
• Amino Acids (analysis)
• Chemical Phenomena
• Chemistry
• Female
• Ganglioneuroma (analysis)
• Humans
• Infant
• Radioimmunoassay
• Retroperitoneal Neoplasms (analysis)
• Vasoactive Intestinal Peptide (isolation & purification)
• Vipoma (analysis)