The influence of diabetes mellitus and hypercorticism on the wound healing of experimental myocardial infarction in rats.

Abstract

In diabetic and glucocorticoid-treated rats myocardial infarction is produced by coronary artery ligation. After the ligation was performed the animals were given 3H-thymidine or 3H-proline at different times. The following parameters were determined: number of DNA- and tropocollagen-synthesizing connective-tissue cells both at the infarction border and infarction site; mean silver-grain density above the nuclei or cells; number of mitoses. The labelling and mitotic indices as well as the standard deviation (in percent) from the mean values were calculated. The following results were obtained: 1. The retarded formation of collagen fibres in diabetic animals is caused by a reduced number of tropocollagen-synthesizing fibroblasts and by a diminished synthesizing performance of the individual cells. 2. Glucocorticoids have a pronounced inhibitory effect on granulation-tissue formation, The 3H--thymidine indices are strikingly low. The synthesis of collagen precursors in the fibroblasts is reduced. The release of tropocollagen from the connective-tissue cells is slowed down.

• Authors: D Kranz, A Hecht, I Fuhrmann, U Keim
• Journal: Experimentelle Pathologie (Exp Pathol (Jena)) 1977 Jul-Aug Vol. 14 Issue 1-2 Pg. 1-8 ISSN: 0014-4908 [Print] Germany
• PMID: 598451 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Tropocollagen
• Collagen
• DNA
• Prednisolone

Topics

• Animals
• Collagen (biosynthesis)
• Connective Tissue (pathology)
• DNA (biosynthesis)
• Diabetes Mellitus, Experimental (metabolism, pathology)
• Male
• Mitosis
• Myocardial Infarction (drug therapy, metabolism, pathology)
• Prednisolone (therapeutic use)
• Rats
• Tropocollagen (biosynthesis)