Determination of estrogen receptors in canine mammary tumors.

Dextran-coated charcoal assay with Scatchard plotting was used for the quantitative determination of high-affinity estradiol receptors (ER) in 10 canine mammary tumor cytosols. Mammary tumors diagnosed histopathologically as adenocarcinomas appeared to have a higher incidence of ER than those diagnosed as mixed mammary tumors. These receptors have a high affinity for estradiol with dissociation constants as determined by Scatchard analysis ranging from 1.2 to 2.0 X 10(-11) M. These dissociation constants are similar to those previously reported for the receptor-estradio interaction from target tissues in other species. The specific binding of the receptor for the hormone was diminished in the presence of an antiestrogenic compound. Our data suggest that canine mammary tumors with significant ER levels would respond favorably to antiestrogen therapy. With the demonstration of ER sites in canine mammary adenocarcinomas as presented herein, the potential for chemotherapeutic management appears to be encouraging.
AuthorsK R Monson, J O Malbica, Hubben
JournalAmerican journal of veterinary research (Am J Vet Res) Vol. 38 Issue 12 Pg. 1937-9 (Dec 1977) ISSN: 0002-9645 [Print] UNITED STATES
PMID596691 (Publication Type: Journal Article)
Chemical References
  • Receptors, Estrogen
  • Estradiol
  • Adenocarcinoma (metabolism, veterinary)
  • Animals
  • Cytosol (metabolism)
  • Dog Diseases (metabolism)
  • Dogs
  • Estradiol (metabolism)
  • Female
  • Mammary Glands, Animal (metabolism)
  • Methods
  • Neoplasms (veterinary)
  • Receptors, Estrogen (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: