Kim, Ki-Han (Wayne State University, Detroit, Mich.). Properties and distribution of intracellular
putrescine in a Pseudomonas. J. Bacteriol. 91:193-197. 1966.-A Pseudomonas species which contains
putrescine as the only intracellular
polyamine was used to study the distribution of
putrescine in the cells and the changes in
putrescine content upon
nitrogen or
carbon and
nitrogen starvation. In the cell-free extract, approximately 80 to 90% of the
putrescine was found in the soluble fraction, and the rest was found in the ribosomal fraction; 50% of the
putrescine could be removed from the cells by
nitrogen starvation.
Putrescine content in the ribosomes prepared from
nitrogen-starved cells was about one-half of that in the unstarved cells.
Putrescine was found in both 30S and 50S ribosomal particles. In the presence of 10(-3)m Mg(++), the ribosomal particles did not exchange bound
putrescine for free
putrescine, but did incorporate free
spermine from the medium. Cells grown on glucose-NH(3) medium contained large amounts of acetyl
putrescine. Cells grown on
putrescine contained negligible amounts of acetyl
putrescine, but readily formed acetyl
putrescine when subjected to
starvation.