Immunogenicity and role of size: response of guinea pigs to oligotyrosine and tyrosine derivatives.

Abstract

Guinea pigs injected with 100 micrograms of p-azobenzenearsonate derivatives of hexa-L-tyrosine, tri-L-tyrosine, or N-acetyl-L-tyrosine amide, in complete Freund's adjuvant, developed, after 10 to 19 days, delayed-type hypersensitivity to these substances. This was shown by skin reactions, followed by the formation of circulating antibodies that were detectable by passive cutaneous anaphylaxis. Experiments with p-azobenzenearsonate-hexa-L-tyrosine labeledwith iodine-131 showed that this substance was bound in vitro to proteins of normal guinea pig serum. Binding was similar with the nonantigenic hexa-L-tyrosine and its p-azobenzoate derivative.

• Authors: F Borek, Y Stupp, M Sela
• Journal: Science (New York, N.Y.) (Science) Vol. 150 Issue 3700 Pg. 1177-8 (Nov 26 1965) ISSN: 0036-8075 [Print] United States
• PMID: 5852973 (Publication Type: Journal Article)

Chemical References

• Antigens
• Iodine Isotopes
• Peptides
• Serum Albumin

Topics

• Animals
• Antibody Formation
• Antigens (pharmacology)
• Guinea Pigs
• Hypersensitivity, Delayed (etiology)
• Immunochemistry
• In Vitro Techniques
• Iodine Isotopes
• Molecular Weight
• Passive Cutaneous Anaphylaxis
• Peptides (pharmacology)
• Protein Binding
• Serum Albumin