[Biosynthesis of enniatin by washed cells of Fusarium sambucinum].

Abstract

Biosynthesis of the depsipeptide membrane ionophore--enniatin B by the washed mycelium Fusarium sambucinum Fuck 52 377 was studied. Metabolic precursors of enniatin B, alpha-ketovaleric acid, 14C-L-valine, and 14CH3-methionine, were added to the system after starvation. The amino acid content in the metabolic pool increased 1.5 times after addition of alpha-ketovaleric acid, 2.2 times after that of valine, and 2.5 times after addition of methionine. 14C-L-valine and 14CH3-methionine were incorporated into the molecule of enniatin B. Valine methylation in the molecule occurred at the level of synthesized depsipeptide. Amino acids of the metabolic pool performed the regulatory function in the synthesis.

• Authors: A E Minasian, D N Chermenskĭ, A M Bezborodov
• Journal: Prikladnaia biokhimiia i mikrobiologiia (Prikl Biokhim Mikrobiol) 1979 Sep-Oct Vol. 15 Issue 5 Pg. 654-9 ISSN: 0555-1099 [Print] Russia (Federation)
• Vernacular Title: Biosintez enniatina otmytymi kletkami Fusarium Sambucinum.
• PMID: 583180 (Publication Type: English Abstract, Journal Article)

Chemical References

• Anti-Bacterial Agents
• Carbon Radioisotopes
• Valerates
• Methionine
• Valine

Topics

• Anti-Bacterial Agents (biosynthesis)
• Carbon Radioisotopes
• Fusarium (metabolism)
• Kinetics
• Methionine (metabolism)
• Valerates (metabolism)
• Valine (metabolism)