[Phosphoric acid ester poisoned rats after antidote therapy. 2. Determination of serum enzyme activity].

Abstract

Paraoxon in doses of one LD50 (0.426 mg/kg), eight times and eighty times LD 50 was applied s.c. to female Sprague-Dawley rats. After 3, 6, 10, 24 and 36--48 h the activities of enzymes GOT, GPT, GLDH, SDH, CPK and ChE were measured, once after i.m. antidote application of Toxogonin only, of Toxogonin + atropine and the next one after application of combination Toxogonin + atropine + Solcoseryl (low-molecular components of deproteinized blood from young calves. The values obtained showed that in spite of treatment with Toxogonin or Toxogonin + atropine the activities of the enzymes increased; this enhancement could be prevented by addition of Solcoseryl to Toxogonin + atropine. The ChE-activity after 36 h was equivalent to that of the control value. The effect of paraoxon in the initial phase of poisoning was discussed in connection with hypoxia and acidosis resulting from a respiratory insufficiency as well as the inhibition of ATPase-activity with restriction of the energy metabolism following: consequently the effect of Solcoseryl was interpreted as an activation of the disturbed energy metabolism.

• Authors: H Hettwer, L Gandawidjaja
• Journal: Arzneimittel-Forschung (Arzneimittelforschung) Vol. 28 Issue 8 Pg. 1381-8 ( 1978) ISSN: 0004-4172 [Print] Germany
• Vernacular Title: Befunde an Phosphorsäureester-vergifteten Ratten nach Antidottherapie. II. Aktivitätsbestimmungen einiger Serumenzyme.
• PMID: 582404 (Publication Type: English Abstract, Journal Article)

Chemical References

• Enzymes
• Tissue Extracts
• Actihaemyl
• L-Lactate Dehydrogenase
• Succinate Dehydrogenase
• Aspartate Aminotransferases
• Alanine Transaminase
• Creatine Kinase
• Acetylcholinesterase
• Paraoxon

Topics

• Acetylcholinesterase (blood)
• Actihaemyl (pharmacology)
• Alanine Transaminase (blood)
• Animals
• Aspartate Aminotransferases (blood)
• Creatine Kinase (blood)
• Enzymes (blood)
• Female
• L-Lactate Dehydrogenase (blood)
• Lethal Dose 50
• Paraoxon (poisoning)
• Rats
• Succinate Dehydrogenase (blood)
• Time Factors
• Tissue Extracts (pharmacology)