Purification and concentration of influenza types A and B by chromatography on calcium phosphate.

Abstract

A rapid chromatographic method for the isolation of types A and B influenza virus from allantoic fluid was described. The adsorbent was prepared from calcium dihydrogen orthophosphate monohydrate [Ca(H(2)PO(4))(2).H(2)O] by alkali treatment. The addition of sodium trimetaphosphate to the influenza-infected allantoic fluid afforded a 67 to 100% viral recovery and a 26 to 43-fold increase in purity.

• Authors: M Lapidus
• Journal: Applied microbiology (Appl Microbiol) Vol. 17 Issue 4 Pg. 504-6 (Apr 1969) ISSN: 0003-6919 [Print] United States
• PMID: 5772390 (Publication Type: Journal Article)

Chemical References

• Calcium Phosphates

Topics

• Animals
• Calcium Phosphates
• Chick Embryo
• Chromatography
• Methods
• Orthomyxoviridae (isolation & purification)