Cytoplasmic structures associated with an arbovirus infection: loci of viral ribonucleic acid synthesis.

Abstract

Unique cytoplasmic structures, herein designated as type I cytopathic vacuoles (CPV-I), are found in chick embryo cells early in the logarithmic phase of Semliki Forest virus replication. High resolution autoradiography demonstrated that the CPV-I are loci of (3)H-uridine incorporation. This evidence correlates well with previous biochemical data and electron microscopy of the subcellular fractions active in Semliki Forest virus ribonucleic acid synthesis. Origin of the CPV-I within host cell cytoplasm is confirmed by the distribution of electron-dense tracer particles and sequential ultrastructural observations.

• Authors: P M Grimley, I K Berezesky, R M Friedman
• Journal: Journal of virology (J Virol) Vol. 2 Issue 11 Pg. 1326-38 (Nov 1968) ISSN: 0022-538X [Print] United States
• PMID: 5750316 (Publication Type: Journal Article)

Chemical References

• RNA, Viral
• Tritium
• Thorium Dioxide
• Uridine

Topics

• Animals
• Autoradiography
• Chick Embryo
• Cytoplasm
• Membranes (metabolism)
• Microscopy, Electron
• RNA, Viral (biosynthesis)
• Semliki forest virus (growth & development)
• Thorium Dioxide
• Tritium
• Uridine (metabolism)
• Virus Replication