Abstract |
The urinary protein excreted in heavy-chain disease was separated by ion-exchange chromatography into two broad fractions designated Cra-1 and Cra-2. For a dimeric molecular weight of approx. 51000, Cra-1 contained 3-4 residues of 6-deoxy-l-galactose (l- fucose), 10 of d-mannose, 5-6 of d-galactose, 12 of 2-acetamido-2-deoxy-d-glucose ( N-acetyl-d-glucosamine) and 4-5 of N-acetylneuraminic acid ( sialic acid), whereas the corresponding values for Cra-2 were 2, 10, 7, 12 and 7. Cra-2 contained in addition 1 residue of 2-acetamido-2-deoxy-d-galactose ( N-acetyl-d-galactosamine). Cra-1 contained an average of four oligosaccharide units, two of which contained 1 residue of 6-deoxy-l-galactose, 3 of d-mannose, 1 of d-galactose and 3 of 2-acetamido-2-deoxy-d-glucose, whereas the other two units contained the same proportions of 6-deoxy-l-galactose, d-mannose and 2-acetamido-2-deoxy-d-glucose but 2 residues of d-galactose and 2 of N-acetylneuraminic acid. Cra-2 also contained an average of four oligosaccharide units, but the range of glycopeptides was much wider, containing 0-1 residue of 6-deoxy-l-galactose, 2-3 of d-mannose, 2-3 of d-galactose, 2-3 of 2-acetamido-2-deoxy-d-glucose and 1-3 of N-acetylneuraminic acid. Possible reasons for this heterogeneity are discussed. Glycopeptides were also isolated from Cra-2 that contained 1 residue of d-mannose, 2 of d-galactose, 1 of 2-acetamido-2-deoxy-d-galactose and 0-3 of N-acetylneuraminic acid.
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Authors | J R Clamp, G Dawson, E C Franklin |
Journal | The Biochemical journal
(Biochem J)
Vol. 110
Issue 3
Pg. 385-93
(Dec 1968)
ISSN: 0264-6021 [Print] England |
PMID | 5701662
(Publication Type: Journal Article)
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Chemical References |
- Hexosamines
- Neuraminic Acids
- Peptides
- Glucosamine
- Mannose
- Galactose
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Topics |
- Chromatography, Ion Exchange
- Galactose
(analysis)
- Glucosamine
(analysis)
- Heavy Chain Disease
(urine)
- Hexosamines
(analysis)
- Humans
- Mannose
(analysis)
- Molecular Weight
- Neuraminic Acids
(analysis)
- Peptides
(analysis)
- Proteinuria
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