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Intracellular protein breakdown in non-growing cells of Escherichia coli.

Abstract
1. When Escherichia coli leu(-) was incubated at 35 degrees in a medium based on minimal medium, but with the omission of phosphate ions, or glucose, or NH(4) (+) ions and leucine, intracellular protein was degraded at a rate of about 5%/hr. in each case. If Mg(2+) ions were omitted, however, the rate of degradation was 2.9%/hr. 2. Under certain conditions of incubation, protein degradation was inhibited. The inhibitor was neither NH(4) (+) ions nor amino acids, and its properties were not those of a protein, but it might be an unstable species of RNA. 3. Although a large part of the cell protein was degraded at about 5%/hr. during starvation of NH(4) (+) ions and leucine, some proteins were lost at more rapid rates, whereas others were lost at lower rates or not at all. 4. In particular, beta-galactosidase activity was lost at about 8%/hr. during starvation of NH(4) (+) ions and leucine, whereas d-serine-deaminase and alkaline-phosphatase activities were stable. During starvation of Mg(2+) ions, all three enzyme activities were stable.
AuthorsN S Willetts
JournalThe Biochemical journal (Biochem J) Vol. 103 Issue 2 Pg. 453-61 (May 1967) ISSN: 0264-6021 [Print] ENGLAND
PMID5340366 (Publication Type: Journal Article)
Chemical References
  • Amino Acids
  • Bacterial Proteins
  • Carbon Isotopes
  • Nucleosides
  • Phosphates
  • Quaternary Ammonium Compounds
  • RNA
  • Chloramphenicol
  • Alkaline Phosphatase
  • Galactosidases
  • Hydro-Lyases
  • Leucine
  • Valine
  • Magnesium
  • Glucose
Topics
  • Alkaline Phosphatase (metabolism)
  • Amino Acids (analysis)
  • Bacterial Proteins (metabolism)
  • Bacteriological Techniques
  • Bacteriolysis
  • Carbon Isotopes
  • Chloramphenicol (pharmacology)
  • Enzyme Induction
  • Escherichia coli (growth & development, metabolism)
  • Galactosidases (metabolism)
  • Glucose (metabolism)
  • Hydro-Lyases (metabolism)
  • Kinetics
  • Leucine (metabolism)
  • Magnesium (metabolism)
  • Nucleosides
  • Phosphates (metabolism)
  • Quaternary Ammonium Compounds (pharmacology)
  • RNA (pharmacology)
  • Ultrasonics
  • Valine (analysis)

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