Abstract |
The influence on red blood cells (RBC) of two cancerostatic-( ZIMET 3106 and ZIMET 3393) and two immunomodulating agents ( ZIMET 3164 and ZIMET 86/76) administered subcutaneously, or intraperitoneally in case of the radiolabelled N- mustard compounds, to mice, was studied by means of measurements of the radiolabelled compounds in blood, by cell electrophoresis, filipin-induced hemolysis of pretreated RBC and by scanning electronmicroscopy. The portion of doses applied which binds to RBC-surface decreases in the sequence of ZIMET 3106, ZIMET 3164, ZIMET 3393 and ZIMET 86/76. However, the highest reduction of the electrophoretic mobility and protection from filipin-induced hemolysis of pretreated RBC was found with ZIMET 3164. In both tests ZIMET 86/76 proved to be ineffective. These findings are in good agreement with the modifications demonstrated in pretreated RBC after filipin-induced hemolysis by means of SEM. The results presented point to a N-mustard specific interaction between the cholesterol of the membrane and/or other membrane constituents. However, these fundamental differences found in binding affinity, surface-charges and membrane interactions caused by the chemically similar compounds cannot be attributed merely to the N-mustard group.
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Authors | K Augsten, T Peschke, K Wohlrabe |
Journal | Experimentelle Pathologie
(Exp Pathol (Jena))
Vol. 17
Issue 7-8
Pg. 380-6
( 1979)
ISSN: 0014-4908 [Print] Germany |
PMID | 510451
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Benzimidazoles
- Immunosuppressive Agents
- Nitrogen Mustard Compounds
- Quinolinium Compounds
- Filipin
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Benzimidazoles
(metabolism, pharmacology)
- Electrophoresis
- Erythrocyte Membrane
(drug effects, ultrastructure)
- Erythrocytes
(drug effects)
- Filipin
(pharmacology)
- Hemolysis
- Immunosuppressive Agents
(metabolism)
- Male
- Mice
- Microscopy, Electron, Scanning
- Nitrogen Mustard Compounds
(metabolism, pharmacology)
- Quinolinium Compounds
(metabolism, pharmacology)
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