On the structure of cytolipin R, a ceramide tetrahexoside hapten from rat lymphosarcoma.

Abstract

Cytolipin R, a ceramide tetrahexoside isolated from rat lymphosarcoma, was studied by sequential hydrolysis with specific glycosidases which revealed the anomeric configurations of the glycosidic bonds. Sugar linkages were established by combined gas-liquid chromatography and mass spectrometry of the partially methylated alditol acetates prepared after permethylation and hydrolysis of the intact lipid. Results indicated the structure of cytolipin R to be N-acetylgalactosaminyl(beta1-->3)galactosyl(alpha1-->3) galactosyl(beta1-->4)glucosyl ceramide. Cytolipin K (globoside I) differs in having a -galactosyl(alpha1-->4)galactosyl- internal linkage, and this difference must account for the immunological differences between cytolipin K and cytolipin R.

• Authors: R Laine, C C Sweeley, Y T Li, A Kisic, M M Rapport
• Journal: Journal of lipid research (J Lipid Res) Vol. 13 Issue 4 Pg. 519-24 (Jul 1972) ISSN: 0022-2275 [Print] United States
• PMID: 5068000 (Publication Type: Journal Article)

Chemical References

• Cerebrosides
• Haptens
• Galactosamine
• Galactosidases
• Hexosaminidases
• Galactose

Topics

• Alkylation
• Animals
• Cerebrosides (analysis, isolation & purification)
• Chemical Phenomena
• Chemistry
• Chromatography, Gas
• Computers
• Galactosamine (analysis)
• Galactose (analysis)
• Galactosidases
• Haptens
• Hexosaminidases
• Isomerism
• Lymphoma, Non-Hodgkin (immunology)
• Mass Spectrometry
• Plants (enzymology)
• Rats
• Sarcoma, Experimental (immunology)
• Structure-Activity Relationship