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Radio-immunoassay of gastrin in human plasma.

Abstract
1. A radio-immunoassay for gastrin has been developed using partially purified porcine gastrin to raise antibodies and highly purified natural porcine gastrin I for radio-iodination with (125)I. The separation of antibody-bound from free hormone was performed by a double-antibody method.2. In this assay highly purified natural porcine gastrin I, synthetic human gastrin I, radio-iodinated porcine gastrin I, gastrin in the plasma of a healthy volunteer, a patient with pernicious anaemia and another patient with the Zollinger-Ellison syndrome were immunologically identical.3. The fasting plasma gastrin concentration of fourteen gastric ulcer patients was significantly higher than that of the 113 hospital controls with no history of gastro-intestinal disease, while twenty-seven duodenal ulcer patients had gastrin levels within the normal range.4. Plasma gastrin concentration was significantly elevated in pernicious anaemia (fifty-one patients), achlorhydria (thirty-three patients), hypochlorhydria (eleven patients) and in nine patients with histologically proven Zollinger-Ellison syndrome.5. In human volunteers a protein meal stimulated endogenous gastrin release while a carbohydrate meal did not. Atropine sulphate I.M., and hydrochloric acid orally, produced a significant fall in the level of circulating gastrin.
AuthorsP C Ganguli, W M Hunter
JournalThe Journal of physiology (J Physiol) Vol. 220 Issue 2 Pg. 499-510 (Jan 1972) ISSN: 0022-3751 [Print] England
PMID5014108 (Publication Type: Journal Article)
Chemical References
  • Dietary Proteins
  • Gastrins
  • Iodine Isotopes
  • Sucrose
  • Atropine
  • Hydrochloric Acid
Topics
  • Achlorhydria (blood)
  • Anemia, Pernicious (blood)
  • Animals
  • Atropine (pharmacology)
  • Dietary Proteins
  • Duodenal Ulcer (blood)
  • Gastrins (blood, metabolism)
  • Guinea Pigs
  • Humans
  • Hydrochloric Acid (pharmacology)
  • Iodine Isotopes
  • Methods
  • Rabbits
  • Radioimmunoassay
  • Stimulation, Chemical
  • Stomach Ulcer (blood)
  • Sucrose (pharmacology)
  • Swine
  • Zollinger-Ellison Syndrome (blood)

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