Use of chromosomal translocations with in situ DNA hybridisation to confirm localisation of human 5S ribosomal RNA genes.

Abstract

Two cases of chromosomal translocations involving the long arm of chromosome 1 were investigated for 5S ribosomal gene localisation using in situ hybridisation. In the first family, there was an interstitial translocation of 1q25-32 to chromosome 5; the 5S genes remained on chromosome 1. In the second family, there was a translocation of 1q42-44 to chromosome 21q12; the 5S gene locus in this case was translocated. This shows that the 5S ribosomal genes are at position 1q42-44, confirming a previous assignment based on adenovirus-induced uncoiling and on a partial trisomy (Steffensen et al., 1977).

• Authors: S J Fennell, S Malcolm, R Williamson, M A Ferguson-Smith
• Journal: Journal of medical genetics (J Med Genet) Vol. 16 Issue 4 Pg. 246-53 (Aug 1979) ISSN: 0022-2593 [Print] England
• PMID: 490577 (Publication Type: Journal Article)

Chemical References

• RNA, Ribosomal

Topics

• Chromosome Banding
• Chromosome Mapping
• Chromosomes, Human, 1-3 (ultrastructure)
• Chromosomes, Human, 21-22 and Y (ultrastructure)
• Chromosomes, Human, 4-5 (ultrastructure)
• Female
• Humans
• Hybridization, Genetic
• Male
• RNA, Ribosomal (genetics)
• Translocation, Genetic