Normal immunosuppressive protein purification and quantitative estimation experiments.

Abstract

An improved method for the preparation and purification of normal immunosuppressive protein (NIP) is described. The purified material has a molecular weight between 10,000 and 25,000. Its biological and serological activity is approximately 10--20 times higher than that of the crude fraction. An antibody to normal immunosuppressive protein prepared in rabbits made the quantitative estimation of NIP by a haemaggluination inhibition test possible. Similarly, a very sensitive assay for the quantitative determination of NIP by its inhibitory effect on the proliferation of EL-4 tumor cells is also described. Eluates prepared from polyacrylamide gels were active in inhibiting EL-4 tumor cell proliferation and neutralized the anti-NIP activity in the haemagglutination inhibition test.

• Authors: D Nelken, R Goren, H Ovadia, N Hanna
• Journal: Journal of immunological methods (J Immunol Methods) Vol. 28 Issue 3-4 Pg. 267-76 ( 1979) ISSN: 0022-1759 [Print] Netherlands
• PMID: 479607 (Publication Type: Journal Article)

Chemical References

• Blood Proteins
• Immune Sera
• Immunosuppressive Agents

Topics

• Animals
• Blood Proteins (immunology, isolation & purification)
• Electrophoresis, Polyacrylamide Gel
• Hemagglutination Inhibition Tests
• Humans
• Immune Sera (pharmacology)
• Immunosuppressive Agents (immunology, isolation & purification)
• Molecular Weight
• Neoplasms, Experimental (immunology)
• Rabbits