Abstract |
The Crabtree effect can be observed when the O2 consumption of tumour cells or of mammalian cells grown in culture is measured in physiological medium containing glucose. The effect of 2 hypoxic cell radiosensitizers, misonidazole and NDPP, on the O2 consumption of Ehrlich ascites tumour cells was compared in media with and without glucose. A stimulatory effect on O2 consumption was found for 5--20mM misonidazole as well as for 0.5mM NDPP, both in media containing 10(-2)M glucose. Thus glucose induced a Crabtree effect in Ehrlich tumour cells, expressed as 38--45% inhibition of O2 consumption relative to that in the same medium without glucose. The stimulatory effect of misonidazole and NDPP on O2 utilization in medium with glucose undoubtedly appeared as a release from the Crabtree effect.
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Authors | I Mustea, A Bara |
Journal | British journal of cancer
(Br J Cancer)
Vol. 40
Issue 2
Pg. 295-9
(Aug 1979)
ISSN: 0007-0920 [Print] England |
PMID | 475972
(Publication Type: Journal Article)
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Chemical References |
- Nitro Compounds
- Nitroimidazoles
- Propiophenones
- Radiation-Sensitizing Agents
- 4-nitro-3-dimethylaminopropiophenone
- Misonidazole
- Glucose
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Topics |
- Animals
- Carcinoma, Ehrlich Tumor
(metabolism)
- Cells, Cultured
- Glucose
(pharmacology)
- Mice
- Misonidazole
(pharmacology)
- Nitro Compounds
- Nitroimidazoles
(pharmacology)
- Oxygen Consumption
- Propiophenones
(pharmacology)
- Radiation-Sensitizing Agents
(pharmacology)
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