Abstract |
Numerous lines of mouse lymphoid tumors (13 of 22 tested) showed, with increased sensitivity, a property of normal mouse splenic lymphocytes, the potential for growth promotion in vitro by specific thiols added to standard culture media. For lymphoma L1210 (V), structure activity relationships were examined; 9 of 30 thiols promoted growth; the most active was alpha-thioglycerol, effective at 0.2 microM. Thiols became oxidized under conditions of tissue culture and had half-lives of less than 8 h. Disulfides of active thiols promoted growth of lymphoma cells. The mitogenic response of splenic lymphocytes to lectins was increased by thiols- disulfides which promoted the growth of lymphoma cells, but the response varied with the mitogen preparation used and under some conditions thiols- disulfides were inhibitory.
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Authors | J D Broome, M W Jeng |
Journal | The Journal of experimental medicine
(J Exp Med)
Vol. 138
Issue 3
Pg. 574-92
(Sep 01 1973)
ISSN: 0022-1007 [Print] United States |
PMID | 4727914
(Publication Type: Journal Article)
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Chemical References |
- Disulfides
- Mitogens
- Sulfhydryl Compounds
- Mercaptoethanol
- Cysteine
- Thymidine
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Topics |
- Animals
- Cell Line
- Cells, Cultured
- Cysteine
(pharmacology)
- Disulfides
(pharmacology)
- Half-Life
- Leukemia L1210
- Lymphocytes
(drug effects)
- Lymphoma
- Mercaptoethanol
(pharmacology)
- Mice
- Mitogens
(pharmacology)
- Mitosis
(drug effects)
- Spleen
(cytology)
- Structure-Activity Relationship
- Sulfhydryl Compounds
(pharmacology)
- Thymidine
(metabolism)
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