Presumptive identification of group A, B, and D streptococci.

Abstract

A battery of five tests was used for presumptive identification of the pathogenic streptococci. The non-serological methods included determination of hemolysis for all strains, bacitracin susceptibility for group A streptococci, hippurate hydrolysis by group B streptococci, and bile-esculin reaction for group D streptococci. Enterococcal group D streptococci were differentiated from non-enterococcal group D streptococci by 6.5% NaCl tolerance. Two other categories of streptococci resulted: beta-hemolytic streptococci non-groups A, B, or D; and alpha- or nonhemolytic streptococci, not enterococci, not further identified (viridans streptococci). The tests were used as a battery and not as single entities. In this manner more than 99% of the group A, 99% of the group B, 81% of the beta-hemolytic streptococci non-group A, B, or D, 99% of the group D enterococci, 97% of the group D non-enterococci, and 94% of the viridans streptococci were correctly identified.

• Authors: R R Facklam, J F Padula, L G Thacker, E C Wortham, B J Sconyers
• Journal: Applied microbiology (Appl Microbiol) Vol. 27 Issue 1 Pg. 107-13 (Jan 1974) ISSN: 0003-6919 [Print] United States
• PMID: 4589119 (Publication Type: Journal Article)

Chemical References

• Flavonoids
• Hippurates
• Bacitracin
• Sodium Chloride

Topics

• Bacitracin (pharmacology)
• Bacteriological Techniques
• Bile (metabolism)
• Drug Resistance, Microbial
• Flavonoids (metabolism)
• Hemolysis
• Hippurates (metabolism)
• Hydrolysis
• Methods
• Microbial Sensitivity Tests
• Serotyping
• Sodium Chloride (pharmacology)
• Streptococcus (classification, drug effects, immunology, metabolism, pathogenicity)