Effect of endotoxin on tumor resistance in mice.

Abstract

As reported earlier, an intraperitoneal injection of 1 mug of endotoxin (ET) from Serratia marcescens rendered mice resistant against the nonspecific mouse ascites tumor TA3-Ha upon challenge 24 h after pretreatment with ET. Further studies were aimed at the elaboration of conditions which achieved maximal resistance. It appears that (i) a 10-mug dose of ET was approximately the optimal dose for protection; (ii) pretreatment with ET 3 to 0 days prior to tumor challenge gave best protection; and (iii) the intravenous injection of ET showed a lower protection against the tumor than intraperitoneal application. Studies on the mechanism of ET protection indicate that (i) ET does not have a direct cytotoxic effect on tumor cells; (ii) normal spleen cells exposed to ET in vitro can adoptively transfer protection against tumor; and (iii) spleen cells activated in vivo by intravenous injection of ET can adoptively transfer protection. The possible involvement of mononuclear cells is discussed.

• Authors: C Yang, A Nowotny
• Journal: Infection and immunity (Infect Immun) Vol. 9 Issue 1 Pg. 95-100 (Jan 1974) ISSN: 0019-9567 [Print] United States
• PMID: 4587387 (Publication Type: Journal Article)

Chemical References

• Endotoxins
• Tissue Extracts
• Tritium
• Thymidine

Topics

• Animals
• Carcinoma, Ehrlich Tumor (immunology)
• Cell Survival
• Dose-Response Relationship, Drug
• Endotoxins (administration & dosage, pharmacology)
• Female
• Immunization, Passive
• In Vitro Techniques
• Injections, Intraperitoneal
• Injections, Intravenous
• Lymphocyte Activation (drug effects)
• Lymphocytes (drug effects)
• Mice
• Mice, Inbred C57BL
• Mollusca
• Neoplasm Transplantation
• Serratia marcescens (immunology)
• Spleen (cytology)
• Thymidine (metabolism)
• Time Factors
• Tissue Extracts
• Tritium