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Translation of bacteriophage Q RNA by cytoplasmic extracts of mammalian cells.

Abstract
Cytoplasmic extracts from Krebs II mouse ascites cells and from L cells translate messenger RNA from coliphage Qbeta with fidelity to produce products that migrate on polyacrylamide gels with those products directed by Qbeta RNA in an Escherichia coli cell-free system. The mammalian cell extracts correctly initiate and terminate Qbeta coat protein synthesis, as shown by: (i) [(3)H]lysine-and [(3)H]arginine-labeled tryptic peptides derived from the coat-sized product resemble these from authentic Qbeta coat protein, (ii) Qbeta coat (which contains methionine only at the N-terminal end) can be radioactively labeled with methionine only if the methionine is formylated, and (iii) L cell extracts directed by Qbeta am(-)11 (an amber mutant in the coat protein) RNA make no completed coat-sized material, but do make a peptide the size of the authentic amber coat fragment.
AuthorsT G Morrison, H F Lodish
JournalProceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 70 Issue 2 Pg. 315-9 (Feb 1973) ISSN: 0027-8424 [Print] United States
PMID4568725 (Publication Type: Journal Article)
Chemical References
  • Carbon Isotopes
  • Peptides
  • RNA, Messenger
  • RNA, Viral
  • Sulfur Isotopes
  • Viral Proteins
  • Tritium
  • Trypsin
Topics
  • Animals
  • Carbon Isotopes
  • Carcinoma, Krebs 2 (metabolism)
  • Cell-Free System
  • Cells, Cultured
  • Coliphages (analysis)
  • Cytoplasm
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli (metabolism)
  • Female
  • Kinetics
  • L Cells (metabolism)
  • Mice
  • Peptides (analysis)
  • Protein Biosynthesis
  • RNA, Messenger (metabolism)
  • RNA, Viral (isolation & purification, metabolism)
  • Sulfur Isotopes
  • Tritium
  • Trypsin (metabolism)
  • Viral Proteins (analysis, biosynthesis, metabolism)

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