Abstract |
Cytoplasmic extracts from Krebs II mouse ascites cells and from L cells translate messenger RNA from coliphage Qbeta with fidelity to produce products that migrate on polyacrylamide gels with those products directed by Qbeta RNA in an Escherichia coli cell-free system. The mammalian cell extracts correctly initiate and terminate Qbeta coat protein synthesis, as shown by: (i) [(3)H] lysine-and [(3)H] arginine-labeled tryptic peptides derived from the coat-sized product resemble these from authentic Qbeta coat protein, (ii) Qbeta coat (which contains methionine only at the N-terminal end) can be radioactively labeled with methionine only if the methionine is formylated, and (iii) L cell extracts directed by Qbeta am(-)11 (an amber mutant in the coat protein) RNA make no completed coat-sized material, but do make a peptide the size of the authentic amber coat fragment.
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Authors | T G Morrison, H F Lodish |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 70
Issue 2
Pg. 315-9
(Feb 1973)
ISSN: 0027-8424 [Print] United States |
PMID | 4568725
(Publication Type: Journal Article)
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Chemical References |
- Carbon Isotopes
- Peptides
- RNA, Messenger
- RNA, Viral
- Sulfur Isotopes
- Viral Proteins
- Tritium
- Trypsin
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Topics |
- Animals
- Carbon Isotopes
- Carcinoma, Krebs 2
(metabolism)
- Cell-Free System
- Cells, Cultured
- Coliphages
(analysis)
- Cytoplasm
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
(metabolism)
- Female
- Kinetics
- L Cells
(metabolism)
- Mice
- Peptides
(analysis)
- Protein Biosynthesis
- RNA, Messenger
(metabolism)
- RNA, Viral
(isolation & purification, metabolism)
- Sulfur Isotopes
- Tritium
- Trypsin
(metabolism)
- Viral Proteins
(analysis, biosynthesis, metabolism)
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