Abstract |
Glucosamine 6-phosphate synthase (EC 5.3.1.19) purified from various rat tissues by a procedure involving chromatography on diethylaminoethyl Sephadex and hydroxylapatite were characterized by means of isoelectric focusing. The non-hepatic isozyme, previously reported to be present in Yoshida sarcoma, has a pI of 4.1 and is distinguished from the hepatic isozyme, with a pI of 5.0. The pI 4.1 form is the major one in all of the fast-growing, transplantable hepatomas studied. Although not detectable in 19-day fetal liver or normal adult liver, the pI 4.1 form has been observed in the whole 12-day fetus and adult brain as almost the sole form of glucosamine 6-phosphate synthase.
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Authors | T Miyagi, S Tsuiki |
Journal | Cancer research
(Cancer Res)
Vol. 39
Issue 7 Pt 1
Pg. 2779-82
(Jul 1979)
ISSN: 0008-5472 [Print] United States |
PMID | 445481
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
- Carbohydrate Epimerases
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Topics |
- Animals
- Brain
(enzymology)
- Carbohydrate Epimerases
(metabolism)
- Fetus
- Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)
(metabolism)
- Isoelectric Focusing
- Liver
(enzymology)
- Liver Neoplasms, Experimental
(enzymology)
- Male
- Rats
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