Preventive effects of phylloquinone on hemorrhagic death induced by butylated hydroxytoluene in male rats.

Abstract

The effects of vitamin K on hemorrhagic death induced by dietary butylated hydroxytoluene (BHT) were studied. Male Sprague-Dawley rats were given BHT or two phenolic antioxidants (2,4,6-tri-tert-butylphenol and 2,5-di-tert-butylhydroquinone) in combination with a 24% casein basal diet. The levels of the phenols were chosen to nearly equal LD50 (40 days). Hemorrhagic death, hemorrhage and a decrease in prothrombin index caused by 1.20% BHT were prevented by simultaneously adding phylloquinone (0.68 mumole/kg/day). Phylloquinone also inhibited the effect of the related phenolic antioxidants. Ten nanomoles of phylloquinone injected into the femoral vein on day 3 of feeding 1.2% BHT increased the prothrombin concentration from 28% of normal to 100% of normal within 18 to 24 hours. Phylloquinone oxide also prevented hypoprothrombinemia due to BHT. These results suggest that BHT-induced hemorrhagic death may be caused by direct and/or indirect vitamin K deficiency, and its mechanism may be different from those of warfarin.

• Authors: O Takahashi, K Hiraga
• Journal: The Journal of nutrition (J Nutr) Vol. 109 Issue 3 Pg. 453-7 (Mar 1979) ISSN: 0022-3166 [Print] United States
• PMID: 430246 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Cresols
• Hydroquinones
• Phenols
• Butylated Hydroxytoluene
• 2,5-di-tert-butylhydroquinone
• Vitamin K 1
• 2,4,6-tri-tert-butylphenol

Topics

• Animals
• Antioxidants
• Butylated Hydroxytoluene
• Cresols
• Diet
• Dose-Response Relationship, Drug
• Hemorrhage (chemically induced, mortality, prevention & control)
• Hydroquinones
• Hypoprothrombinemias (chemically induced, prevention & control)
• Injections, Intravenous
• Male
• Phenols
• Prothrombin Time
• Rats
• Vitamin K 1 (administration & dosage, therapeutic use)