Effect of poxvirus infection on host cell deoxyribonucleic acid synthesis.

Abstract

Deoxyribonucleic acid (DNA) synthesis was studied in poxvirus-infected cells by measuring (14)C-thymidine incorporation into viral and host cell DNA. A complete separation of the two species of DNA was achieved by combining the previously used "Dounce method" with a separation method based on different reannealing properties of viral and vertebrate DNA. Shortly after infection of HeLa cells with poxviruses, a burst of viral DNA synthesis occurred in the cytoplasm, but a rapid inhibition of host-cell DNA synthesis in the nucleus was observed. This inhibition of cellular DNA synthesis was also found if an accumulation of viral DNA was prevented. At high multiplicites, ultraviolet-irradiated virus inhibited host-cell DNA synthesis to the same extent as fully infectious poxvirus. Under the same conditions, heating at 60 C for 15 min caused a decrease in the ability of cowpox virus to inhibit host-cell DNA synthesis, but did not produce the same effect on vaccinia virus strain WR.

• Authors: C Jungwirth, J Launer
• Journal: Journal of virology (J Virol) Vol. 2 Issue 5 Pg. 401-8 (May 1968) ISSN: 0022-538X [Print] United States
• PMID: 4301310 (Publication Type: Journal Article)

Chemical References

• Carbon Isotopes
• DNA, Viral
• DNA
• Thymidine

Topics

• Carbon Isotopes
• Cell Nucleus (metabolism)
• Cytoplasm (metabolism)
• DNA (analysis, biosynthesis)
• DNA, Viral (analysis)
• Female
• HeLa Cells
• Hot Temperature
• Humans
• Methods
• Poxviridae (radiation effects)
• Poxviridae Infections (metabolism)
• Radiation Effects
• Thymidine (metabolism)
• Ultraviolet Rays