Response of two strains of L5178Y cells to cis-dichlorobis(cyclopentylamine)platinum(II). I. Cross-sensitivity to cis-PAD and UV light.

Abstract

The response to cis-dichlorobis(cyclopentylamine)platinum(II) (cis-PAD) an antitumour platinum complex, was studied in two strains of murine lymphoma L5178Y cross-sensitive to X-rays and UV light. Dose-survival relationship, DNA synthesis formation of chromatid aberrations, progression through the cell cycle, and growth and viability changes after 1 h cis-PA; treatment at 37 degree C were examined and compared with the effects of X-rays and UV light. In both strains, cis-PAD caused immediate inhibition of progression through the cell cycle, reduced rate of DNA synthesis, delayed appearance of chromatid aberrations, and delayed death; however, there is a marked difference in sensitivity to cis-PAD between L5178Y-S strain (D0 approx. 5.8 microgram/ml) and L5178Y-R strain (D0 approx. 2.5 microgram/ml). In both strains a close resemblance was found between dose-survival relationships after cis-PAD and UV light treatment, respectively.

• Authors: I Szumiel
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 24 Issue 1 Pg. 51-72 (Jan 1979) ISSN: 0009-2797 [Print] Ireland
• PMID: 428002 (Publication Type: Comparative Study, Journal Article)

Chemical References

• DNA, Neoplasm
• Organoplatinum Compounds
• dichlorobis(cyclopentylamine)platinum

Topics

• Animals
• Cell Cycle (drug effects, radiation effects)
• Cell Division (drug effects, radiation effects)
• Cell Survival (drug effects, radiation effects)
• Chromosome Aberrations
• DNA, Neoplasm (biosynthesis)
• L Cells (drug effects, metabolism, radiation effects)
• Mice
• Organoplatinum Compounds (pharmacology)
• Ultraviolet Rays
• X-Rays