Detection and localization of virus-specific DNA by in situ hybridization of cells during infection and rapid transformation by the murine sarcoma-leukemia virus.

Abstract

Cytological preparations of interphase nuclei and chromosomes from mouse 3T6 cells prepared at various times after infection with the murine sarcomaleukemia virus complex were hybridized with the [(3)H]DNA product of the viral RNA-directed DNA polymerase. While uninfected nuclei had an average of 4 autoradiographic grains, infected nuclei had 30 grains at 5 hr after infection and 63-65 grains at 11 and 25 hr. Virus-specific grains were localized in the chromocenters of interphase nuclei and were found also in the centromeric heterochromatin region of metaphase chromosomes. These findings provide evidence that the viral RNA-directed DNA polymerase functions to synthesize virus-specific DNA early after infection and that newly synthesized viral DNA rapidly becomes associated with or integrated into specific intranuclear sites.

• Authors: M C Loni, M Green
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 71 Issue 9 Pg. 3418-22 (Sep 1974) ISSN: 0027-8424 [Print] United States
• PMID: 4139710 (Publication Type: Journal Article)

Chemical References

• DNA, Viral
• Heterochromatin
• Tritium
• RNA-Directed DNA Polymerase

Topics

• Animals
• Autoradiography
• Cell Nucleus (analysis)
• Cell Transformation, Neoplastic
• Cells, Cultured
• Chromosomes (analysis)
• DNA, Viral (analysis, biosynthesis)
• Gammaretrovirus
• Heterochromatin (analysis)
• Karyotyping
• Leukemia Virus, Murine
• Mice
• Nucleic Acid Hybridization
• RNA-Directed DNA Polymerase
• Sarcoma (microbiology)
• Time Factors
• Tritium