Group B arbovirus structural and nonstructural antigens. II. Purification of Saint Louis encephalitis virus intracellular antigens.

Abstract

Three serologically distinct antigens were identified in homogenates of Saint Louis encephalitis (SLE) virus-infected cells after Brij-58 solubilization and diethylaminoethyl (DEAE)-cellulose chromatography. These antigens were designated as antigen I, II, and III. Immunodiffusion analyses showed that all antigen peaks which eluted from the DEAE-cellulose column contained the intracellular major envelope protein, antigen I. This protein was the only viral antigen which eluted at 0.125 M KCl in DEAE-cellulose column peak C. Optical density peak D, which eluted at 0.2 M KCl, contained both antigens I and III. Antigen III was purified from this column fraction approximately 200-fold by organic solvent extraction and chromatography on hydroxylapatite and Sepharose 6B. Purified antigen III had a molecular weight of 80,000 to 85,000 and contained only one antigenic determinant as judged by immunodiffusion analysis.

• Authors: A A Qureshi, D W Trent
• Journal: Infection and immunity (Infect Immun) Vol. 8 Issue 6 Pg. 985-92 (Dec 1973) ISSN: 0019-9567 [Print] United States
• PMID: 4131772 (Publication Type: Journal Article)

Chemical References

• Antigens, Viral
• Carbon Radioisotopes
• Epitopes
• Hydroxyapatites
• Surface-Active Agents
• Viral Proteins
• Tritium
• Leucine

Topics

• Animals
• Antigens, Viral (analysis, isolation & purification)
• Carbon Radioisotopes
• Chromatography
• Chromatography, DEAE-Cellulose
• Chromatography, Gel
• Complement Fixation Tests
• Culture Techniques
• Electrophoresis, Polyacrylamide Gel
• Encephalitis Virus, St. Louis (analysis, immunology)
• Encephalitis Viruses (immunology)
• Epitopes
• Hydroxyapatites
• Immunodiffusion
• Kidney
• Leucine
• Molecular Weight
• Surface-Active Agents
• Swine
• Tritium
• Viral Proteins (analysis)
• Virus Cultivation