[Titration of the antibodies to the Crimean hemorrhagic fever virus in a drop of the cell suspension from infected tissue cultures by means of indirect immunofluorescence].

Abstract

A comparatively simple method has been developed and tested for the detection and titration of antibody to Crimean hemorrhagic fever virus (CHF) by indirect immunofluorescence on slides with previously prepared acetone-inactivated virus in drops of cell suspension from infected BHK-21 and 6619 cell cultures. The antibody titers determined by the indirect immunofluorescence procedures were 4- and 8-fold higher than those determined by the complement-fixation test. This opens the possibility of using non-infectious antigens of cell suspensions for detection or titration by the FA procedure of antibody to CHF virus which may be of importance to diagnostic laboratories which have no conditions for work with live virus.

• Authors: G N Zgurskaia, M P Chumakov
• Journal: Voprosy virusologii (Vopr Virusol) 1977 Sep-Oct Issue 5 Pg. 606-8 ISSN: 0507-4088 [Print] Russia (Federation)
• Vernacular Title: Titrovanie amtitel k virusu krysmskoĭ gemorragicheskoĭ likhoradki v kaple vzvesi kletok iz zarazhennykh kul'tur tkani metodom nepriamoĭ immunofliuorestsentsii.
• PMID: 412325 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Antibodies, Viral
• Suspensions

Topics

• Antibodies, Viral (isolation & purification)
• Arboviruses (immunology)
• Complement Fixation Tests
• Culture Techniques
• Fluorescent Antibody Technique
• Hemorrhagic Fever Virus, Crimean-Congo (immunology)
• Suspensions
• Virus Cultivation