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Concentration and purification of hepatitis B antigen with polyethylene glycol and polyelectrolyte 60, a cross-linked copolymer of isobutylene maleic anhydride.

Abstract
A simple two-step procedure is described for the concentration and purification of hepatitis B antigen. The antigen is precipitated by polyethylene glycol and then adsorbed on and eluted from an insoluble polyelectrolyte, polyelectrolyte 60, which is a cross-linked copolymer of isobutylene maleic anhydride.
AuthorsE De Rizzo, R Pandey, C Wallis, J L Melnick
JournalInfection and immunity (Infect Immun) Vol. 6 Issue 3 Pg. 335-8 (Sep 1972) ISSN: 0019-9567 [Print] United States
PMID4118047 (Publication Type: Journal Article)
Chemical References
  • Albumins
  • Alkenes
  • Anhydrides
  • Blood Proteins
  • Glycols
  • Hepatitis B Antigens
  • Maleates
  • Polyethylenes
  • Polymers
  • gamma-Globulins
  • polyelectrolyte 60
Topics
  • Adsorption
  • Albumins (analysis)
  • Alkenes
  • Anhydrides
  • Blood Protein Electrophoresis
  • Blood Proteins (analysis)
  • Chemical Phenomena
  • Chemistry
  • Glycols
  • Hepatitis B Antigens (isolation & purification)
  • Humans
  • Immunoelectrophoresis
  • Maleates
  • Polyethylenes
  • Polymers
  • gamma-Globulins (analysis)

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