Properties of maedi nucleic acid and the presence of ribonucleic acid- and deoxyribonucleic acid-dependent deoxyribonucleic acid polymerase in the virions.

Abstract

Maedi virus contains a ribonucleic acid (RNA) which can be resolved into three major components, namely, 62S, 33S, and 13S, by sucrose gradient centrifugation. The presence of RNA- and deoxyribonucleic acid (DNA)-dependent DNA polymerase in virions of maedi virus was demonstrated. The enzyme product could be converted into acid-soluble form by pancreatic deoxyribonuclease, but was resistant to digestion by pancreatic ribonuclease and to hydrolysis by NaOH.

• Authors: F H Lin, H Thormar
• Journal: Journal of virology (J Virol) Vol. 10 Issue 2 Pg. 228-33 (Aug 1972) ISSN: 0022-538X [Print] United States
• PMID: 4116344 (Publication Type: Journal Article)

Chemical References

• DNA, Viral
• RNA, Viral
• Thymine Nucleotides
• Tritium
• Sodium Hydroxide
• RNA-Directed DNA Polymerase
• Ribonucleases
• Thymidine
• Uridine

Topics

• Animals
• Centrifugation, Density Gradient
• Choroid Plexus
• Culture Techniques
• DNA, Viral (biosynthesis)
• Hydrolysis
• Pulmonary Adenomatosis, Ovine (microbiology)
• RNA, Viral (analysis, isolation & purification)
• RNA-Directed DNA Polymerase (analysis, metabolism)
• Ribonucleases
• Sheep
• Sodium Hydroxide
• Thymidine (metabolism)
• Thymine Nucleotides (metabolism)
• Tritium
• Uridine (metabolism)
• Viruses, Unclassified (analysis, enzymology, growth & development, metabolism)