Branching of central neurons: intracellular cobalt injection for light and electron microscopy.

Abstract

Cobalt chloride can be injected into an identified nerve cell body in an insect ganglion and reacted with ammonium sulfide to stain the soma and its branches with a black precipitate. The stained cell body and its branches throughout the neuropil are visible in both the light and electron microscope. In whole mount preparations, the resolution of neurites within the neuropil is of a quality that permits the comparison of branching patterns between cells and during various functional states.

• Authors: R M Pitman, C D Tweedle, M J Cohen
• Journal: Science (New York, N.Y.) (Science) Vol. 176 Issue 4033 Pg. 412-4 (Apr 28 1972) ISSN: 0036-8075 [Print] United States
• PMID: 4112670 (Publication Type: Journal Article)

Chemical References

• Cobalt

Topics

• Action Potentials
• Animals
• Cobalt
• Cockroaches
• Electric Stimulation
• Ganglia (cytology)
• Methods
• Microscopy
• Microscopy, Electron
• Neurons (cytology, physiology)
• Staining and Labeling