High-performance liquid chromatographic method for monitoring leupeptin in mouse serum and muscle by pre-column fluorescence derivatization with benzoin.

Abstract

A high-performance liquid chromatographic method is described for the determination of leupeptin, a possible therapeutic drug for muscular dystrophy, in mouse serum and muscle. Leupeptin is reduced with sodium borohydride to leupeptinol, and then converted to a fluorescent derivative with benzoin. The derivative is separated on a reversed-phase column (LiChrosorb RP-18) with isocratic elution and determined with fluorescence detection. The detection limits of leupeptin in serum and muscle are 250 pmol/ml (107 ng/ml) and 500 pmol/g (214 ng/g), respectively, corresponding to approximately 150 fmol each in a 100-microliters injection volume. This method is simple and sensitive enough to permit the quantification of leupeptin in biological samples from mice dosed with leupeptin.

• Authors: M Kai, T Miura, J Ishida, Y Ohkura
• Journal: Journal of chromatography (J Chromatogr) Vol. 345 Issue 2 Pg. 259-65 (Dec 13 1985) Netherlands
• PMID: 4086597 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Leupeptins
• Oligopeptides
• leupeptin
• Benzoin

Topics

• Animals
• Benzoin
• Chromatography, High Pressure Liquid
• Leupeptins (analysis, blood, isolation & purification)
• Mice
• Mice, Inbred C57BL
• Muscles (analysis)
• Oligopeptides (analysis)
• Spectrometry, Fluorescence