HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Preparation of tuberculin antigen L.

Abstract
The preparation of antigen L using culture filtrates of live BCG is described. The procedure consisted essentially of precipitating this filtrate with 10% trichloroacetic acid in the presence of N-butyl alcohol 4% final concentration. The precipitate was chromatographed on carboxy-methyl-trisacryl-M and then on DEAE-trisacryl-M; in both cases, maximal biological activity (delayed-type hypersensitivity) was found in the void volume. The product thus obtained was chromatographed on a molecular sieve so as to eliminate poorly active fractions. The final product was 30-fold more active on guinea-pigs sensitized with live BCG than on those sensitized with inactivated BCG.
AuthorsF Romain, E Versmisse, P Pescher, J Augier
JournalAnnales de l'Institut Pasteur. Microbiologie (Ann Inst Pasteur Microbiol (1985)) 1985 Sep-Oct Vol. 136B Issue 2 Pg. 235-48 Netherlands
PMID4083836 (Publication Type: Journal Article)
Chemical References
  • Antigens, Bacterial
  • Tuberculin
  • antigen L
Topics
  • Animals
  • Antigens, Bacterial (immunology, isolation & purification)
  • Chromatography, DEAE-Cellulose
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Electrophoresis, Polyacrylamide Gel
  • Guinea Pigs
  • Isoelectric Focusing
  • Tuberculin (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: