Abstract |
The preparation of antigen L using culture filtrates of live BCG is described. The procedure consisted essentially of precipitating this filtrate with 10% trichloroacetic acid in the presence of N-butyl alcohol 4% final concentration. The precipitate was chromatographed on carboxy-methyl-trisacryl-M and then on DEAE-trisacryl-M; in both cases, maximal biological activity (delayed-type hypersensitivity) was found in the void volume. The product thus obtained was chromatographed on a molecular sieve so as to eliminate poorly active fractions. The final product was 30-fold more active on guinea-pigs sensitized with live BCG than on those sensitized with inactivated BCG.
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Authors | F Romain, E Versmisse, P Pescher, J Augier |
Journal | Annales de l'Institut Pasteur. Microbiologie
(Ann Inst Pasteur Microbiol (1985))
1985 Sep-Oct
Vol. 136B
Issue 2
Pg. 235-48 Netherlands |
PMID | 4083836
(Publication Type: Journal Article)
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Chemical References |
- Antigens, Bacterial
- Tuberculin
- antigen L
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Topics |
- Animals
- Antigens, Bacterial
(immunology, isolation & purification)
- Chromatography, DEAE-Cellulose
- Chromatography, Gel
- Chromatography, Ion Exchange
- Electrophoresis, Polyacrylamide Gel
- Guinea Pigs
- Isoelectric Focusing
- Tuberculin
(immunology)
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