Activation of K-ras and oncogenes other than ras family in rat fibrosarcomas induced by 1,8-dinitropyrene.

Abstract

Oncogenes of fibrosarcomas of rats, induced by subcutaneous injection of 1,8-dinitropyrene (1,8-DNP), were examined by NIH 3T3 cell transfection assay and Southern blot analysis. Transformants containing rat specific repetitive sequences were obtained with DNAs of 4 fibrosarcomas, 1,8-DNP1, 1,8-DNP2, 1,8-DNP3 and 1,8-DNP7. A transformant, 1,8-DNP2-2, induced by DNA of a fibrosarcoma, 1,8-DNP2, and 7 secondary transformants derived from it contained rat K-ras sequences. Another transformant, 1,8-DNP2-1, induced by the same sarcoma did not have a ras family oncogene. This indicates that the sarcoma, 1,8-DNP2, has at least 2 transforming genes. The transforming genes of 6 other transformants derived from 3 sarcomas did not contain ras family or neu transforming genes.

• Authors: M Ochiai, M Nagao, T Tahira, F Ishikawa, K Hayashi, H Ohgaki, M Terada, N Tsuchida, T Sugimura
• Journal: Cancer letters (Cancer Lett) Vol. 29 Issue 2 Pg. 119-25 (Nov 1985) ISSN: 0304-3835 [Print] Ireland
• PMID: 4075282 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• Pyrenes
• 1,8-dinitropyrene

Topics

• Animals
• Cell Line
• DNA, Neoplasm (analysis)
• Electrophoresis, Polyacrylamide Gel
• Fibrosarcoma (chemically induced, genetics)
• Male
• Nucleic Acid Hybridization
• Oncogenes
• Pyrenes
• Rats
• Rats, Inbred F344
• Repetitive Sequences, Nucleic Acid