Abstract |
Indirect immunoperoxidase staining by the peroxidase-antiperoxidase (PAP) technique was carried out on 23 human primary intracranial germ-cell tumors (17 germinomas, one embryonal carcinoma, one yolk-sac tumor, three teratomas, and one teratoma with embryonal carcinoma) and on six human primary pineal non- germ-cell tumors (one pineocytoma, two pineoblastomas, two astrocytomas, and one glioblastoma multiforme). The technique used specific rabbit antisera against placental alkaline phosphatase (PLAP), alpha-fetoprotein (AFP), and human chorionic gonadotropin (HCG). Thirteen of 17 intracranial germinomas (76.5%) showed positive staining for PLAP mainly on the tumor cell membrane. In six primary intracranial non-seminomatous germ-cell tumors, there was weak positive staining indicating the presence of PLAP in only a few cells of one embryonal carcinoma, and in some glandular epithelial cells of one teratoma; this staining was limited to the cytoplasm. None of the other six primary pineal non- germ-cell tumors showed any positive PLAP reaction. From these results, PLAP was shown to be very useful in histopathology as a diagnostic tumor marker of intracranial germinoma. Positive AFP staining was seen in several yolk-sac tumor cells and a few embryonal carcinoma cells. However, no intracranial germinomas and non- germ-cell tumors of the pineal region showed positive reaction. As for HCG, only one suprasellar germinoma and one pineal embryonal carcinoma among 29 specimens contained a few positive-staining tumor cells.
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Authors | J Shinoda, Y Miwa, N Sakai, H Yamada, H Shima, K Kato, M Takahashi, K Shimokawa |
Journal | Journal of neurosurgery
(J Neurosurg)
Vol. 63
Issue 5
Pg. 733-9
(Nov 1985)
ISSN: 0022-3085 [Print] United States |
PMID | 4056875
(Publication Type: Journal Article)
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Chemical References |
- GPI-Linked Proteins
- Isoenzymes
- Alkaline Phosphatase
- alkaline phosphatase, placental
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Topics |
- Adolescent
- Adult
- Alkaline Phosphatase
- Brain Neoplasms
(analysis, pathology)
- Child
- Dysgerminoma
(analysis, pathology)
- Female
- GPI-Linked Proteins
- Glioma
(analysis, pathology)
- Humans
- Immunochemistry
- Isoenzymes
(analysis)
- Male
- Pinealoma
(analysis, pathology)
- Teratoma
(analysis, pathology)
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