Abstract |
A change of pH did not modify the sensitivity of aequorin to Ca2+, but an increase of pH enhanced the Ca2+ sensitivity of the myofilaments of a skinned canine cardiac Purkinje cell. The tension-pCa curve did not present any hysteresis when a given [free Ca2+] was reached from a higher versus from a lower [free Ca2+] in the presence of pH 6.60, 7.10 or 7.40. A rapid variation of pH in either direction failed to induce Ca2+ release from the sarcoplasmic reticulum (SR). The proton ionophores CCCP and gramicidin also failed to induce Ca2+ release from the SR. Increase of pH from 7.10 to 7.40 enhanced Ca2+ accumulation into the SR and, thereby, augmented the Ca2+ content of the SR. Consequently, the amplitude of a subsequent Ca2+ release triggered by a rapid increase of [free Ca2+] at the outer surface of the SR was increased. Conversely, a decrease of pH from 7.10 to 6.60 diminished the Ca2+ accumulation into the SR, the Ca2+ content of the SR and the amplitude of a subsequent Ca2+-induced release of Ca2+ from the SR. In addition, the optimum [free Ca2+] for triggering Ca2+-induced release of Ca2+ was shifted to higher [free Ca2+] values by a decrease of pH from 7.40 to 7.10 or 7.10 to 6.60. This may help to explain the enhancement of the aequorin light transient during acidosis in the intact cardiac muscle inasmuch as acidosis may increase the [free Ca2+] trigger at the outer surface of the SR by inhibiting Na+-Ca2+ exchange across the sarcolemma.
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Authors | A Fabiato |
Journal | Cell calcium
(Cell Calcium)
Vol. 6
Issue 1-2
Pg. 95-108
(Apr 1985)
ISSN: 0143-4160 [Print] Netherlands |
PMID | 4040434
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Ionophores
- Luminescent Proteins
- Aequorin
- Calcium
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Topics |
- Aequorin
- Animals
- Calcium
(analysis, metabolism)
- Cell Compartmentation
- Cell Membrane Permeability
- Cytoplasm
(metabolism)
- Cytoskeleton
(physiology)
- Dogs
- Heart
(physiology)
- Hydrogen-Ion Concentration
- In Vitro Techniques
- Ionophores
(pharmacology)
- Luminescent Proteins
- Myocardial Contraction
- Myocardium
(metabolism)
- Sarcoplasmic Reticulum
(metabolism)
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