Urokinase-type plasminogen activator in ascites obtained from the patient with mammary cancer.

Abstract

Plasminogen activator (ascites PA) was purified from the ascites of the patient with breast cancer using lysine-Sepharose, salting out, DEAE Sephadex A-50 and anti-urokinase (UK) Sepharose chromatography. The ascites PA was adsorbed on anti-UK Sepharose and its activity was neutralized by anti-UK IgG fraction. The molecular weights of the ascites PA proteins were 70,000, 32,000 and 25,000 daltons. The ascites PA activated plasminogen and showed amidolytic activity for S-2444. Zymograms of ascites PA showed that 70,000 and 32,000 daltons proteins had a PA activity. The ascites PA thus contained two types of PAs, one was similar to LMW-UK and the other was an UK type tissue PA.

• Authors: S Hayashi, K Yamada
• Journal: Thrombosis research (Thromb Res) Vol. 38 Issue 5 Pg. 459-67 (Jun 01 1985) ISSN: 0049-3848 [Print] United States
• PMID: 4040279 (Publication Type: Journal Article)

Chemical References

• Plasminogen Activators
• Urokinase-Type Plasminogen Activator

Topics

• Ascites (enzymology)
• Breast Neoplasms (enzymology)
• Female
• Humans
• Immunochemistry
• Middle Aged
• Molecular Weight
• Plasminogen Activators (isolation & purification)
• Urokinase-Type Plasminogen Activator (immunology, isolation & purification)