Elevated phosphate transporting activity and phosphate carrier content in mitochondria of rat hepatoma with high glycolytic capacity.

Abstract

Transport of inorganic phosphate into Zajdela hepatoma mitochondria proceeds with approximately the same Km and about two times higher Vmax than the transport into mitochondria of rat liver. As detected by (a) titration of the inhibition of mitochondrial phosphate-stimulated respiration and phosphate-induced swelling by mersalyl and (b) binding of /14C/-NEM and /14C/-DCCD to a 33 kDa protein in mitochondria, the higher phosphate transporting activity of the hepatoma mitochondria is due to about a three fold increase in phosphate carrier content in the tumor mitochondria.

• Authors: E Tkácová, S Kuzela
• Journal: Biochemistry international (Biochem Int) Vol. 11 Issue 1 Pg. 45-50 (Jul 1985) ISSN: 0158-5231 [Print] Australia
• PMID: 4038318 (Publication Type: Journal Article)

Chemical References

• Carrier Proteins
• Phosphate-Binding Proteins
• Phosphates

Topics

• Animals
• Biological Transport
• Carrier Proteins (metabolism)
• Glycolysis
• In Vitro Techniques
• Kinetics
• Liver Neoplasms, Experimental (metabolism)
• Mitochondria, Liver (metabolism)
• Phosphate-Binding Proteins
• Phosphates (metabolism)
• Rats