[Met-enkephalin-Arg-Gly-Leu-like immunoreactivity-containing nerve fibers and cell bodies in the abdominal sympathetic ganglia of the rat--an immunohistochemical study].

Abstract

The occurrence and localization of Met-enkephalin-Arg-Gly-Leu (Met-Enk-Arg-Gly-Leu)-like immunoreactivity in the lumbar paravertebral ganglion and the superior mesenteric ganglion in the rat were investigated by immunocytochemistry. Colchicine was employed as an axonal flow blocker to accumulate Met-Enk-Arg-Gly-Leu in the nerve cell bodies. The Met-Enk-Arg-Gly-Leu serum (R 0171) was produced by immunizing a mixed breed female rabbit with synthetic Met-Enk-Arg-Gly-Leu conjugated to ascaris protein by the glutaraldehyde method. Sections, 7 micron in thickness, of Bouin-fixed and paraffin-embedded tissues were immunostained by the peroxidase-antiperoxidase (PAP) method. Results obtained were summarized as follows: Colchicine-untreated rats possessed no strongly-immunoreactive nerve cell bodies for Met-Enk-Arg-Gly-Leu in the lumbar paravertebral ganglion, though a few of them were weakly immunopositive. Met-Enk-Arg-Gly-Leu-like immunoreactivity showing nerve fibers were occasionally seen in nerve fiber bundles of the lumbar paravertebral ganglion. In the superior mesenteric ganglion, a dense network of positively immunostained nerve fibers with a beaded appearance were seen around nerve cell bodies which were immunonegative for Met-Enk-Arg-Gly-Leu. In colchicine-treated rats, 55 percent of nerve cell bodies of the lumbar paravertebral ganglion showed various degrees of positive immunoreactivities for Met-Enk-Arg-Gly-Leu. Many strongly immunostained nerve terminals were seen in the lumbar paravertebral ganglion of colchicine-treated rats. In the superior mesenteric ganglion, the immunoreactivity for Met-Enk-Arg-Gly-Leu in the beaded nerve fibers around nerve cell bodies was intensified by the colchicine treatment, whereas the nerve cell bodies themselves remained immunonegative for Met-Enk-Arg-Gly-Leu.

• Authors: T Shimosegawa, M Kobari, M Yambe, M Koizumi, T Toyota, Y Goto, S Kobayashi, T Mochizuki, C Yanaihara, N Yanaihara
• Journal: Nihon Naibunpi Gakkai zasshi (Nihon Naibunpi Gakkai Zasshi) Vol. 61 Issue 5 Pg. 581-91 (May 20 1985) ISSN: 0029-0661 [Print] Japan
• PMID: 4018310 (Publication Type: English Abstract, Journal Article)

Chemical References

• Enkephalin, Methionine
• enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-
• Colchicine

Topics

• Animals
• Colchicine (pharmacology)
• Enkephalin, Methionine (analogs & derivatives, analysis, immunology)
• Ganglia, Sympathetic (analysis, immunology)
• Immunochemistry
• Nerve Fibers (analysis, immunology)
• Rats
• Rats, Inbred Strains