Lactate dehydrogenase isoenzymes in diabetic patients.

Abstract

Lactate dehydrogenase (LDH) isoenzyme profiles in human platelets and the sera of patients with type I and II diabetes mellitus and vascular complications, as well as normal subjects were measured utilizing a recently established, modified micromethod. LDH-3 was the predominating species in platelets (37.5 +/- 3.0%), with LDH-2, 1, 4 and 5 following in decreasing order of concentration. The LDH-3/LDH-4 ratio in platelets varied from 6.2 to 1.38. Type I and type II diabetic patients with vascular complications showed a significantly higher ratio for LDH-3/LDH-4 (3.99 +/- 1.20 for DM I, 2.16 +/- 0.25 for DM II patients) than the mean ratio for normal subjects (1.14 +/- 0.08). This platelet-specific LDH isoenzyme pattern may be the result of frequent in vivo platelet-vessel wall interactions in the diabetic patients whose platelets are known to be hyperaggregable in in vitro test systems. Since non-diabetic patients patients with vascular complications also displayed a similarly elevated LDH-3/LDH-4 ratio, a wider classification is preferable, although the measurement of the LDH isoenzyme pattern will be helpful in assessing diabetic vascular complications.

• Authors: R M Nair, M P Menon, J Sagel
• Journal: Clinical physiology and biochemistry (Clin Physiol Biochem) Vol. 3 Issue 4 Pg. 157-65 ( 1985) ISSN: 0252-1164 [Print] Germany
• PMID: 4017424 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Isoenzymes
• L-Lactate Dehydrogenase

Topics

• Adult
• Aged
• Blood Platelets (enzymology)
• Chromatography, DEAE-Cellulose
• Diabetes Mellitus (enzymology)
• Diabetic Angiopathies (enzymology)
• Humans
• Hydrogen-Ion Concentration
• Isoenzymes
• L-Lactate Dehydrogenase (blood)
• Middle Aged