Cytolytic activity of cytotoxin isolated from Indian cobra venom against experimental tumor cells.

Abstract

Cytolytic activity of cytotoxin isolated from the venom of the Indian cobra (Naja naja) on experimental tumor cells was far stronger than that on normal cells such as peritoneal exudate cells, spleen cells, and erythrocytes of the rat. The effect on Yoshida sarcoma cells was temperature-dependent, being stronger at 37 degrees C than at 0 degrees C. Intramolecular disulfide linkages and free amino groups in the cytotoxin molecule were shown to be essential for the lytic action on the cell membrane. Yoshida sarcoma cells treated with 0.1 mM N-ethylmaleimide reduced the cytolytic action of the toxin. Antitumor activity of the cytotoxin toward a Yoshida sarcoma inoculated intraperitoneally into a rat was not observed.

• Authors: T Iwaguchi, M Takechi, K Hayashi
• Journal: Biochemistry international (Biochem Int) Vol. 10 Issue 3 Pg. 343-9 (Mar 1985) ISSN: 0158-5231 [Print] Australia
• PMID: 4015662 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Elapid Venoms
• cobra cytotoxin I
• cobra cytotoxin II

Topics

• Animals
• Antineoplastic Agents
• Binding Sites
• Cell Division (drug effects)
• Cell Membrane (drug effects)
• Cell Survival (drug effects)
• Elapid Venoms (pharmacology)
• Female
• Mice
• Neoplasms, Experimental (drug therapy)
• Rats
• Sarcoma, Yoshida (drug therapy)
• Structure-Activity Relationship