Abstract |
Lectin binding to tumor cells in tissue sections of 16 nonmetastatic and 24 metastatic human adenocarcinomas and 5 nonmetastatic and 5 metastatic murine Lewis lung carcinomas (LLCs) was assessed with an avidin- biotin peroxidase technique. In human tumors, Ulex europaeus agglutinin I (UEA I) showed no binding; whereas concanavalin A (Con A), Ricinus communis agglutinin I (RCA I), wheat germ agglutinin (WGA), soybean agglutinin (SBA), and Dolichos biflorus agglutinin (DBA) bound equally to primaries and metastases. However, peanut agglutinin (PNA) bound to less than 5% of cells in 37 of 40 primaries but to greater than 50% of cells in 18 of 24 metastases. In LLC tumors, UEA I and DBA showed no binding; whereas Con A, RCA I, and WGA bound equally to primaries and metastases. SBA bound to greater than 50% of cells in 5 metastases but not to the 5 primaries. There was less than 5% binding of PNA to 10 primary murine tumors after neuraminidase pretreatment of tissue sections but greater than 50% binding in 3 of 5 metastases. These studies indicate, in both human adenocarcinomas and an experimental tumor system, that most tumor cells which metastasize show preferential binding of PNA and SBA.
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Authors | H J Kahn, R Baumal |
Journal | The American journal of pathology
(Am J Pathol)
Vol. 119
Issue 3
Pg. 420-9
(Jun 1985)
ISSN: 0002-9440 [Print] United States |
PMID | 4014433
(Publication Type: Journal Article)
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Chemical References |
- Lectins
- Peanut Agglutinin
- Plant Lectins
- Receptors, Mitogen
- Soybean Proteins
- soybean lectin
- Neuraminidase
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Topics |
- Adenocarcinoma
(immunology)
- Animals
- Binding Sites, Antibody
(analysis, drug effects)
- Female
- Humans
- Lectins
(immunology)
- Lung Neoplasms
(immunology, secondary)
- Lymphatic Metastasis
- Male
- Mice
- Neuraminidase
(pharmacology)
- Peanut Agglutinin
- Plant Lectins
- Receptors, Mitogen
(analysis)
- Soybean Proteins
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