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Induction of autolysis of staphylococci by the basic peptide antibiotics Pep 5 and nisin and their influence on the activity of autolytic enzymes.

Abstract
Pep 5 and nisin are cationic bactericidal peptides which were shown to induce autolysis in Staphylococcus cohnii 22. In contrast to nisin, Pep 5 induced lysis could be stimulated in the presence of glucose. Addition of lipoteichoic acids (LTA) (D-alanine:phosphorus = 0.475:1) inhibited all effects of Pep 5 on susceptible cells in a molar ratio LTA:Pep 5 of 10:1. Treatment of S. cohnii 22 with Pep 5 or nisin for 20 min and subsequent washing with 2.5 M NaCl released autolysin activity. Crude preparations of the hydrolyzing enzymes produced free amino groups as well as polysaccharide fragments from the murein backbone, suggesting the presence of a muramidase or glucosamidase, and endopeptidase or amidase. Both enzyme activities were inhibited by lipoteichoic acid; they could be fully reactivated by addition of Pep 5 in sufficient concentrations. The velocity of hydrolysis was not influenced by nisin, whereas it was doubled in presence of Pep 5. The results are discussed in view of a possible mechanism of induction of lysis by Pep 5 and nisin.
AuthorsG Bierbaum, H G Sahl
JournalArchives of microbiology (Arch Microbiol) Vol. 141 Issue 3 Pg. 249-54 (Apr 1985) ISSN: 0302-8933 [Print] Germany
PMID4004448 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-Bacterial Agents
  • Bacteriocins
  • Lipopolysaccharides
  • Peptides
  • Phosphatidic Acids
  • Teichoic Acids
  • Nisin
  • staphylococcin
  • lipoteichoic acid
Topics
  • Anti-Bacterial Agents (pharmacology)
  • Bacteriocins (pharmacology)
  • Bacteriolysis (drug effects)
  • Lipopolysaccharides
  • Nisin (pharmacology)
  • Peptides (pharmacology)
  • Phosphatidic Acids (pharmacology)
  • Staphylococcus (drug effects, enzymology)
  • Teichoic Acids (pharmacology)

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