[Certain characteristics of the rat liver transketolase].

Abstract

Modification of the method for transketolase purification in the rat liver was used to reveal the existence of two molecular forms of this enzyme. One of the forms does not react to development of avitaminosis in animals caused by oxythiamine in different doses. The method is developed for isolation of the basic transketolase form in the rat liver with the 50-80% yield of activity. Km values of two sites for coenzyme binding on protein do not depend on the extent of holoenzyme reconstruction from apoenzyme.

• Authors: V L Kubyshin, Z V Gorbach
• Journal: Ukrainskii biokhimicheskii zhurnal (1978) (Ukr Biokhim Zh (1978)) 1985 Mar-Apr Vol. 57 Issue 2 Pg. 37-41 ISSN: 0201-8470 [Print] Ukraine
• Vernacular Title: Nekototye kharakteristiki transketolazy pecheni krysy.
• PMID: 4002365 (Publication Type: English Abstract, Journal Article)

Chemical References

• Apoenzymes
• Isoenzymes
• Oxythiamine
• Transketolase

Topics

• Animals
• Apoenzymes (metabolism)
• Isoenzymes (isolation & purification, metabolism)
• Kinetics
• Liver (drug effects, enzymology)
• Oxythiamine (pharmacology)
• Rats
• Transketolase (isolation & purification, metabolism)