Detection of vitamin K deficiency by use of an enzyme-linked immunosorbent assay for circulating abnormal prothrombin.

Abstract

A monoclonal antibody was raised against an abnormal decarboxylated prothrombin by a cell fusion technique. A cell line which produces an IgG1 murine antibody to the abnormal prothrombin, but not to prothrombin, was selected. Using this antibody we developed an enzyme-linked sandwich immunoassay for the abnormal prothrombin. The detection range was 0.5 X 10(-1) approximately 0.5 X 10(-3) micrograms protein of decarboxylated prothrombin and 0.5 approximately 0.5 X 10(-2) micrograms protein of abnormal prothrombin in vitamin K-deficient subjects. This discrepancy is attributable to a heterogeneity of decarboxylated prothrombin, depending on the number of gamma-carboxyglutamic acid residues. The antibody obtained had a higher affinity to a protein possessing less gamma-carboxyglutamic acid residues. The assay system developed may be useful for the detection of vitamin K deficiency, since a severe deficiency may result in less gamma-carboxyglutamic acid residues in the protein.

• Authors: K Motohara, Y Kuroki, H Kan, F Endo, I Matsuda
• Journal: Pediatric research (Pediatr Res) Vol. 19 Issue 4 Pg. 354-7 (Apr 1985) ISSN: 0031-3998 [Print] United States
• PMID: 4000762 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Biomarkers
• Protein Precursors
• acarboxyprothrombin
• 1-Carboxyglutamic Acid
• Prothrombin

Topics

• 1-Carboxyglutamic Acid (analysis)
• Adult
• Antibodies, Monoclonal
• Biomarkers
• Enzyme-Linked Immunosorbent Assay
• Humans
• Infant
• Protein Precursors (analysis, immunology)
• Prothrombin (analysis, immunology)
• Vitamin K Deficiency (blood, diagnosis)