Study of H-Y antigen in abnormal sex determination with monoclonal antibody and an ELISA.

Abstract

A newly developed enzyme-linked immunosorbent assay (ELISA) has been applied to the study of H-Y antigen in cases of XY, XYY, and X,dicY gonadal dysgenesis, testicular feminization syndrome, XXXXY syndrome, and XX true hermaphroditism. Monoclonal H-Y antibody was absorbed with cells from each of eight patients and from normal male and female controls, and then reacted with a plated antigen source in a system subsuming the addition of biotinylated secondary antibody, avidin-biotin-enzyme complex and substrate, and thereby the generation of a color. Positive absorption decreased the reaction, and this allowed sensitive measurement of H-Y phenotype in an electronic optical density reader. The ELISA obviates many of the technical difficulties encountered in complement-mediated cytotoxicity systems and can be used in the study of clinical cases of aberrant sex determination and in the evaluation of current models of the genetics of sex determination.

• Authors: C A Moreira-Filho, S S Wachtel
• Journal: American journal of medical genetics (Am J Med Genet) Vol. 20 Issue 3 Pg. 525-34 (Mar 1985) ISSN: 0148-7299 [Print] United States
• PMID: 3993678 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• H-Y Antigen

Topics

• Adolescent
• Adult
• Androgen-Insensitivity Syndrome (immunology)
• Antibodies, Monoclonal (immunology)
• Child
• Child, Preschool
• Disorders of Sex Development (immunology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Gonadal Dysgenesis (immunology)
• H-Y Antigen (analysis, genetics)
• Humans
• Infant
• Male
• Sex Chromosome Aberrations (immunology)
• Sex Determination Analysis