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Effect of ouabain on amino acid uptake by mouse ascites-tumour cells in the presence of nigericin.

Abstract
Mouse ascites-tumour cells oxidizing lactate, in a modified Ringer solution, concentrated 2-aminoisobutyrate, L-methionine or 2-(methylamino)isobutyrate about 20-fold from a 0.4 mM solution in the presence of 2-3 micrograms of nigericin/mg cellular dry wt. The ionophore increased cellular [Na+] to almost 100 mM when extracellular [Na+] was about 45 mM. Either valinomycin or the two mitochondrial inhibitors oligomycin and antimycin acting together each markedly lowered the extent to which the tumour cells concentrated amino acid, from the above factor of about 20 to roughly 2-fold. Ouabain (1 mM) had a similar effect, and further raised cellular [Na+]. The sodium pump appeared to be closely involved in amino acid uptake under these conditions.
AuthorsE Johnson, A A Eddy
JournalThe Biochemical journal (Biochem J) Vol. 226 Issue 3 Pg. 773-9 (Mar 15 1985) ISSN: 0264-6021 [Print] England
PMID3985945 (Publication Type: Journal Article)
Chemical References
  • Amino Acids
  • Aminoisobutyric Acids
  • Anti-Bacterial Agents
  • Oligomycins
  • Serum Albumin
  • antimycin
  • 2-aminoisobutyric acid
  • Valinomycin
  • Ouabain
  • Antimycin A
  • Sodium
  • Nigericin
  • Potassium
Topics
  • Amino Acids (metabolism)
  • Aminoisobutyric Acids (metabolism)
  • Animals
  • Anti-Bacterial Agents (pharmacology)
  • Antimycin A (analogs & derivatives, pharmacology)
  • Carcinoma, Ehrlich Tumor (metabolism)
  • In Vitro Techniques
  • Mice
  • Nigericin (pharmacology)
  • Oligomycins (pharmacology)
  • Ouabain (pharmacology)
  • Potassium (metabolism)
  • Serum Albumin (pharmacology)
  • Sodium (metabolism)
  • Valinomycin (pharmacology)

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