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The ribonuclease-III-processing site near the 5' end of an RNA precursor of bacteriophage T4 and its effect on termination.

Abstract
Infection of RNase III- (rnc) Escherichia coli cells with bacteriophage T4 delta 27, a deletion mutant missing seven out of the ten genes in the tRNA transcription unit, results in the accumulation of a tRNA precursor (10.5-S RNA) that contains the sequences of tRNAGln, tRNALeu and species 1 RNA [Pragai and Apirion (1981) J. Mol. Biol. 153, 619-630]. In vitro studies, using partially purified RNase III or cell extracts and 10.5-S RNA as substrate, have revealed a cleavage site at the 5' side of the molecule. A computerized secondary structure suggests that the RNase III cleavage site can be placed in a small bulge which could be part of a duplex structure and is adjacent to A-A-G and its complementary sequence U-U-U in the same relative relationships found for most RNase III cleavage sites were the adjacent sequences are (A-A-G/U-U-C). Under normal processing conditions (presence of RNase III) the 3' end of the processed intermediate precursors, 10.1-S and p2Sp1 RNAs, is C-U-U-(U1-2)-UOH, which is determined by a stem and loop structure that could serve as a rho-independent termination signal site. However, in the absence of RNase III, the accumulated 10.5-S precursor RNA does not terminate at the same site and its 3' end is shifted a few nucleotides downstream. Thus, RNase III, besides playing a role in processing of 10.5-S RNA, also affects the termination of that molecule, even though both sites, the RNase III cleavage site and the termination site, are about 390 nucleotides apart.
AuthorsM Gurevitz, D Apirion
JournalEuropean journal of biochemistry (Eur J Biochem) Vol. 147 Issue 3 Pg. 581-6 (Mar 15 1985) ISSN: 0014-2956 [Print] England
PMID3979389 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Escherichia coli Proteins
  • Nucleic Acid Precursors
  • RNA, Viral
  • Endoribonucleases
  • Ribonuclease III
  • ribonuclease III, E coli
Topics
  • Binding Sites
  • Chemical Phenomena
  • Chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Endoribonucleases (metabolism)
  • Escherichia coli Proteins
  • Nucleic Acid Conformation
  • Nucleic Acid Precursors (isolation & purification)
  • Peptide Chain Termination, Translational
  • RNA, Viral (biosynthesis)
  • Ribonuclease III
  • T-Phages (genetics)
  • Transcription, Genetic

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