Infection of
RNase III- (rnc) Escherichia coli cells with bacteriophage T4 delta 27, a deletion mutant missing seven out of the ten genes in the
tRNA transcription unit, results in the accumulation of a
tRNA precursor (10.5-S RNA) that contains the sequences of
tRNAGln,
tRNALeu and species 1
RNA [Pragai and Apirion (1981) J. Mol. Biol. 153, 619-630]. In vitro studies, using partially purified
RNase III or
cell extracts and 10.5-S
RNA as substrate, have revealed a cleavage site at the 5' side of the molecule. A computerized secondary structure suggests that the
RNase III cleavage site can be placed in a small bulge which could be part of a duplex structure and is adjacent to A-A-G and its complementary sequence U-U-U in the same relative relationships found for most
RNase III cleavage sites were the adjacent sequences are (A-A-G/U-U-C). Under normal processing conditions (presence of
RNase III) the 3' end of the processed intermediate precursors, 10.1-S and p2Sp1 RNAs, is C-U-U-(U1-2)-UOH, which is determined by a stem and loop structure that could serve as a rho-independent termination signal site. However, in the absence of
RNase III, the accumulated 10.5-S
precursor RNA does not terminate at the same site and its 3' end is shifted a few
nucleotides downstream. Thus,
RNase III, besides playing a role in processing of 10.5-S
RNA, also affects the termination of that molecule, even though both sites, the
RNase III cleavage site and the termination site, are about 390
nucleotides apart.