Race:cultivar-specific induction of enzymes related to phytoalexin biosynthesis in soybean roots following infection with Phytophthora megasperma f. sp. glycinea.

Abstract

Primary roots of soybean [Glycine max (L.), cv Harosoy 63] seedlings were inoculated with zoospores from either race 1 (incompatible, host resistant) or race 3 (compatible, host susceptible) of Phytophthora megasperma f. sp. glycinea (Pmg) and the activities of phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), isoflavone synthase, and dihydroxypterocarpan 6a-hydroxylase related to phytoalexin (glyceollin) biosynthesis, and of glucose-6-phosphate dehydrogenase (Glc-6-PDH) and glutamate dehydrogenase (Glu-DH) were determined at various times after inoculation. About 2-4 h after inoculation with race 1, the activities of PAL, CHS, and pterocarpan 6a-hydroxylase were higher than after inoculation with race 3 and increased considerably thereafter. In contrast, activities of these enzymes in the compatible interaction were equal to or only slightly higher than in the controls over the entire infection period investigated (2-8 h). Isoflavone synthase did not increase until 7 h after inoculation with race 1. There were no significant differences in activities for Glc-6-PDH and Glu-DH between inoculated roots and controls. The results show that infection of soybean roots with zoospores of Pmg race 1 causes a race:cultivar-specific early induction of enzymes involved in glyceollin synthesis, whereas such an induction does not occur with zoospores of race 3. These findings are in agreement with the race:cultivar-specific accumulation of glyceollin in soybean roots reported previously [M. G. Hahn, A. Bonhoff, and H. Grisebach (1985) Plant Physiol. 77, 591-601].

• Authors: A Bonhoff, R Loyal, J Ebel, H Grisebach
• Journal: Archives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 246 Issue 1 Pg. 149-54 (Apr 1986) ISSN: 0003-9861 [Print] United States
• PMID: 3963819 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Extracts
• Sesquiterpenes
• Soybean Proteins
• Terpenes
• Cytochrome P-450 Enzyme System
• Mixed Function Oxygenases
• Glucosephosphate Dehydrogenase
• Oxygenases
• cytochrome P450 CYP93A1 (Glycine max)
• isoflavone synthase
• Glutamate Dehydrogenase
• Acyltransferases
• flavanone synthetase
• Phenylalanine Ammonia-Lyase
• Phytoalexins

Topics

• Acyltransferases (metabolism)
• Chytridiomycota
• Cytochrome P-450 Enzyme System
• Enzyme Induction
• Glucosephosphate Dehydrogenase (metabolism)
• Glutamate Dehydrogenase (metabolism)
• Mixed Function Oxygenases (metabolism)
• Oxygenases (metabolism)
• Phenylalanine Ammonia-Lyase (metabolism)
• Phytophthora
• Plant Diseases
• Plant Extracts (biosynthesis)
• Sesquiterpenes
• Soybean Proteins
• Soybeans (enzymology, metabolism)
• Species Specificity
• Terpenes
• Phytoalexins