Abstract |
Cerebrospinal fluid lymphocytes (CSF-L) and peripheral blood lymphocytes (PBL) from patients with multiple sclerosis (MS) and acute aseptic meningoencephalitis (AM) were cultured without and in the presence of pokeweed mitogen (PWM), a polyclonal B cell activator. IgG, IgA and IgM as well as measles IgG antibody production was measured in 7-day-culture supernatants by enzyme-linked immunosorbent assay. MS CSF-L did not respond with increased Ig production after PWM stimulation, in contrast to AM CSF-L which responded to PWM with a modest increase of production of all 3 Ig classes, especially IgG. PBL responded to PWM with a pronounced production of IgG, IgA and especially IgM, showing no difference between MS, AM and healthy controls. CSF-L from only 1 of 7 patients with MS showed increased measles IgG antibody production after PWM stimulation. The poor response of MS CSF-L might be due to maximal activation of B lymphocytes in vivo, thereby limiting further Ig production after stimulation in vitro.
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Authors | A Henriksson, S Kam-Hansen, P Forsberg, M Grandien |
Journal | Journal of neuroimmunology
(J Neuroimmunol)
Vol. 11
Issue 1
Pg. 15-30
(Mar 1986)
ISSN: 0165-5728 [Print] Netherlands |
PMID | 3944248
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Immunoglobulin A
- Immunoglobulin G
- Immunoglobulin M
- Immunoglobulins
- Pokeweed Mitogens
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Topics |
- Adult
- Antibodies, Viral
(analysis, biosynthesis)
- Cerebrospinal Fluid
(cytology, immunology)
- Female
- Humans
- Immunoglobulin A
(analysis, biosynthesis)
- Immunoglobulin G
(analysis, biosynthesis)
- Immunoglobulin M
(analysis, biosynthesis)
- Immunoglobulins
(analysis, biosynthesis)
- Lymphocyte Activation
(drug effects)
- Lymphocytes
(drug effects, physiology)
- Male
- Measles
(immunology)
- Middle Aged
- Multiple Sclerosis
(cerebrospinal fluid, immunology)
- Pokeweed Mitogens
(pharmacology)
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